Kidneys into the circulation, locally acting RAS is also In addition to the systemic RAS, mainlyĬontrolled by the production and release of renin from the Email: 7 July 2014 revision 17 September 2014 accepted 30 September 2014.Ĭontributes to the development and progression of fibrotic and Olica de Valparaıso, Avenida Universidad No 330, Valparaıso, Chile. The renin–angiotensin system (RAS) plays a major role inīlood pressure and fluid volume control however, it alsoĬorrespondence: Alexis A Gonzalez, Instituto de Quımica, Pontificia Key words: angiotensin II-dependent hypertension, collectingĭuct, prorenin receptor, renin, renin–angiotensin system. With the development of hypertension and kidney disease. May i nduce stimulation of intracellular pathways associated The data suggest that renin–(P)RR interactions Of renin regulation in the collecting ducts and its interaction Review focuses on recent evidence demonstrating the mechanism However, the functionalĬontribution of the (P)RR to enhanced renin activity in the collecting duct and its contribution to the development of hypertension and kidney disease have not been well elucidated. Renin and the (P)RR are augmented in renal tissues ofĪngII-dependent hypertensive rats. Stimulation of (P)RR also activates intracellular pathways related to RR) binds renin or prorenin, enhancing renin activity and fullyĪctivating the biologically inactive prorenin peptide. The recently described (pro)renin receptor ((P) TheĪugmentation of renin synthesis and activity in the collectingĭuct may provide a pathway for additional generation of intrarenal and intratubular AngII formation due to the presence ofĪngiotensinogen substrate and angiotensin-converting enzyme Renin is regulated in the collecting duct remain unclear.
Renin has been extensively studied, the mechanisms by which This effect is mediated by the AngII type I receptor (AT1R), Stimulated despite suppression of juxtaglomerular (JG) renin. Hypertension, collecting duct renin synthesis and secretion are The intrarenal renin–angiotensin system (RAS) plays a critical role in the pathogenesis and progression of hypertensionĪnd kidney disease. *Institute of Chemistry, Pontificia Universidad Catolica de Valparaıso, Valparaıso, Chile, †Department of Physiology,Īnd ‡Tulane Hypertension and Renal Center, Tulane University School of Medicine, New Orleans, LA, USA Renin and the (pro)renin receptor in the renal collecting duct: Role in theĪlexis A Gonzalez* and Minolfa C Prieto†‡ An irregular rise in concentrations after more than 2 h implantation was delayed by antihistamine/antiserotonin.Clinical and Experimental Pharmacology and Physiology (2015) 42, 14–21 The concentrations did not change during this time interval and were reduced by less than 10% by antihistamine/antiserotonin treatment. Total protein concentration in 80 wicks averaged 3.44 (S.D.
0.24) g/100 ml, or 63% of plasma albumin concentration. Implantation of 73 wicks in 24 rats for 35 to 120 min gave an average albumin concentration of 2.10 (S.D. Hemoglobin concentration was less than 0.2 g/100 ml in all but one wick. Total protein, albumin and hemoglobin concentrations were measured in the eluate. After 24 h elution the wick was removed, dried and weighed, allowing calculation of wick fluid volume. Blood-stained wicks, about 1 out of 5, were discarded. The wick was then pulled out, both ends cut off, and the middle part quickly transferred to a tared vial containing 2 ml saline and weighed. A new method has been developed for studying interstitial fluid protein concentration: A 3 cm long and 0.5 mm thick nylon thread was sewn into subcutaneous tissue of ether anesthetized rats and left for equilibration with interstitial fluid for 35 to 240 min.
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